Chromatin studies for chromatin immunoprecipitation (ChIP) or Hi-C (chromosome conformation capture) technologies.Targeted methyl sequencing using hybridization capture technology.Methylome analysis from single-stranded, bisulfite-converted DNA down to picogram inputs, as well as single-cell methylation sequencing ( methyl-seq) applications.Targeted RNA sequencing using hybridization capture technology without the need for ribodepletion or poly(A) mRNA selection.Whole transcriptome sequencing from varying quality of RNA samples ranging between picogram to nanogram input ranges.Metagenomic and metatranscriptomic analysis from complex, highly diverse samples for microbial populations (e.g., soil, gut, skin, etc.).Targeted DNA sequencing from high-quality and low-quality samples using multiplexed PCR panels.Whole exome sequencing from high-quality and low-quality samples using hybridization capture probes (smaller targeted panels for a subset of loci are also available).Whole genome sequencing from high-quality and low-quality samples (e.g., FFPE, cfDNA, ssDNA, etc.) from nanogram down to picogram input ranges.IDT offers a diverse line of predesigned and custom products to support: There are many types of NGS it is most commonly used to evaluate DNA, RNA, DNA-protein interactions, and methylation. The reads go through quality control and are aligned to a reference before being evaluated for significant characteristics. Short-read sequencing generates 100–300 base pair lengths called reads. Illumina® sequencing is performed using sequencing-by-synthesis on a flow cell. Library preparation involves adding adapters, which allow the samples to be indexed (barcoded and identified). This nucleic acid material must be prepared for sequencing by converting it into libraries. The first step is nucleic acid extraction, either DNA or RNA. NGS workflowĪll types of NGS follow a similar workflow. Sequencing thousands of gene fragments simultaneously with NGS reduces time and cost associated with sequencing and increases the coverage quality and data output. Traditional Sanger sequencing determines the sequence of a sample one section at a time. NGS is a high-throughput technology that determines the sequence of a sample all at once by using parallel sequencing. It provides information about organisms in areas as diverse as population genetics, epidemiology, organism identification, genotyping, rare variant identification, cancer and rare disease research, gene editing confirmation, and gene-environment interactions. Target Capture Probe Design & Ordering ToolĭNA sequencing with next generation sequencing (NGS): How it worksĭNA sequencing determines the order of the bases that make up DNA. ![]()
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